Development and use of Biotech Products



1. Letter from DBT dated 4 th may 2020 – No. of biotech tools / activities demonstrated
2. The first PSMC meeting held on 2020 April 29, 30



Special program initiated


Program 1


In 12 units, trials were initiated with crop residue along with poultry waste in daily additions incorporated with the inoculum ( inoculum bacteria isolated from cow dung). Use of the inoculum assisted in arresting the foul smell generated by poultry waste and accelerated the composting process. The following observations were made:

  •   Within 60 days the whole composted matter can be removed from the cabin and it can be prepared for the refill.



Recently experiments were initiated for two different methods

1.  Substrate - Agri crop residue + food/fruit waste

2.  Substitute - Agri crop residue + inoculum


Trials were conducted in 6 units. In both trials while filling the cabin with the above substitutes and after 10/15 days, soldier fly baby maggots were deposited (grown in small units as a facility for soldier flies to lay eggs and  the larvae were transferred into the  units). Positive results were recorded and observed in this method. There was faster size reduction but the quantity /yield of compost was reduced. The matured larvae were collected and given as feed to fish rearing units. This method will considerably reduce the fish-feed production cost as the larvae is rich in protein and is suitable for consumption of the fish species . In the next phase it is being planned to extend and establish this method for waste disposal (domestic + chicken waste from shops).


Program 2


From the beginning of the project experts from Hub centre supported by supplementing biotech tools for accelerated/ rapid composting with the contribution as trials in fixing dosage of inoculum for accelerated /rapid bio digestion. Hub centre supplied the EM Product developed from cow dung and other substrates in the environment lab at Hub centre to 15 farmers and information was collected from them. Trials done in different dosages in different types of crop residue and residue combinations. It was found that the normal composting days reduced to 35/40 days from 60/90 days.


3. Pleurotus eous (Pink Mushroom)


 The pink oyster mushroom (Pleurotus eous),resembles most other oyster mushroom species like Pleurotus florida , except the pink colour, which fades with cooking. The taste is also broadly similar to that of other oysters. The Pink Oyster grows quickly and due to this it is resistant to contamination by moulds. It fruits heavily. Besides the taste and lovely colour, pink oyster mushrooms are a healthy food and may convey certain other health benefits, a possibility supported by modern research.


The pink oyster is a thin, soft, shelf-like shape, with a short, asymmetrical stem or stipe. The bright pink colour generally fades to whitish as the fruiting body ages. Curiously, though the mycelium starts out white, as most fungi do, it quickly turns pink. The spores, which can be numerous, can also be pink. This species is tropical so the Spawn production and cultivation is difficult.


Spore culture technique in the controlled condition was successful and thus we can use it for the cultivation of Pink mushrooms. Because of the nutritional and medicinal contents, it is considered as a nutraceutical (Functional food).



Benefits of Pink Mushroom

  • Nutrition

  • Anti- Oxidant

  • Anti- Bacterial

  • Anti- Cancer

  • Immune System support

  • Vascular Suppor



4. Hypsizygus ulmarius (HU oyster mushroom)


The HU oyster mushroom is an excellent edible mushroom which can be easily grown either for commercial purpose or for home consumption. It is an excellent source of high quality protein and vitamins (especially Vitamin B). The average biological efficiency can be 60-90%. The yield, texture and flavour of this mushroom is far superior as compared to the commercial oyster mushrooms like Pleurotus florida or P. sajor-caju presently being grown in India. Its spore content is very low hence does not cause respiratory allergy problem as the presently grown oyster species. It is highly recommended for stomach and intestinal diseases It was introduced for commercial production for the first time in India by IIHR. Mushrooms initially grey in colour, fading on maturity. Gills and stalks white. Mushrooms large and fleshy with excellent taste. Mushrooms occuring in clusters. Shelf life 36-48 hours at 25-30°C and 4-5 days at 4°C.



To Standardization of cultivation methods • Study of spawn run period, Bud formation, maturity and harvesting

• Total cropping cycle of this variety

• To study the average biological efficiency Results till now • Cultivation has been standardized by sterilization of ( Steam sterilization for 1 hr) paddy straw.

• It completes spawn run in 15-20 days in a temperature range of 25-30°C.

• Bud formation begins after 5-7 days after transferring in to harvesting room and matures for harvest within 2-3 days.


Inhouse R & D works at Hub Centre in association with

Biotech KISAN Project


ZeWa Feeds : Black Soldier Fly (BSF) to reduce food waste


This project helps to reduce global food wastage and provide affordable, high quality feed for livestock farmers. This is achieved by cultivating Black Soldier Fly (Hermetia illucens) Larvae and bio converting food waste into useful products such as poultry feeds, fish feeds and fertilizer. The team developed a few home-based prototypes earlier and up on the success, and they collaborated with Biotech KISAN project  to develop a pilot facility to optimize the process and to develop a scalable model. The pilot plant has been set up by December 2020 and trial feeds are currently being supplied to some selected fish and poultry farmers of acquaintance for further study. If successfully scaled, the method will not only address the growing bio-waste concern, but also provide a great reduction in feed prices which is a major concern for livestock farmers.